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FtsN switches FtsA and FtsQLB to the ON conformations via its cytoplasmic domain (Cyto) and its E domain (E), respectively.
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(B) The signal transduction pathway leading to the activation of FtsWI. Residues altered by mutations studied here are indicated and colored orange. coli FtsW and the catalytic residues of TtRodA and TtPBP2 are colored red. The residues are numbered according to E. TtRodA is colored green and TtPBP2 is colored cyan, the unstructured region in ECL4 of TtRodA is colored orange. thermophilus RodA-PBP2 (PDB ID#: 6P5L) and the location of the corresponding residues in FtsW and FtsI analyzed in this study. This supports a model in which the interaction between the ECL4 of SEDS proteins and the pedestal domain of their cognate bPBPs plays a critical role in the activation mechanism. Mapping these mutations, as well as others affecting the activity of FtsWI, on the RodA-PBP2 structure revealed they are located at the interaction interface between the extracellular loop 4 (ECL4) of FtsW and the pedestal domain of FtsI (PBP3). Epistatic analysis of these mutations and others confirmed that the FtsN-triggered activation signal goes from FtsQLB to FtsI to FtsW. In another approach we isolated suppressors of an inactive FtsL mutant and obtained FtsWE289G and FtsIK211I and found they bypassed FtsN. To try to understand the basis for activation we isolated additional substitutions at this position and found that only the original substitution produced an active mutant whereas drastic changes resulted in an inactive mutant. Previously, we isolated an activation mutation in ftsW (M269I) that allows cell division with reduced FtsN function. In Escherichia coli FtsN switches FtsA and FtsQLB to the active forms that synergize to stimulate FtsWI, but the exact mechanism is not well understood. The activities of these SEDS-bPBPs complexes are tightly regulated to ensure proper cell elongation and division. In the title bar of the Activation Manager, it will show version 1.2.0.3.1 to denote that it is the PBP 3.1 version.SEDS family peptidoglycan (PG) glycosyltransferases, RodA and FtsW, require their cognate transpeptidases PBP2 and FtsI (class B penicillin binding proteins) to synthesize PG along the cell cylinder and at the septum, respectively. Run the PBP 3.1 Activation Manager by clicking Start > PBP 3.1 from ME Labs, Inc.
#PBP3 ACTIVATION KEY UPGRADE#
PBP 3.1 activation keys are obtained by purchasing an upgrade to PBP 3.1.
#PBP3 ACTIVATION KEY ACTIVATION KEY#
If you enter a PBP 3.0 activation key into the PBP 3.1 Activation Manager, it will reject it. Make sure you are running the PBP 3.1 Activation Manager and using a PBP 3.1 activation key.
#PBP3 ACTIVATION KEY SERIAL#
If the message is actually "invalid serial number", it indicates that the PBP Activation Manager is rejecting the key, most likely due to a version mismatch or an incorrectly entered key. If that is the actual message, let me know and I'll investigate further. The error message you've reported ( "registration not valid" ) isn't familiar to me. They are each activated as separate applications with separate activation keys. You can keep both installed, then use the Microcode Compile and Program Options screen to select which compiler to use. The two installations (3.0 and 3.1) should not interfere with one another. If you think a PBP installation is corrupt, just reinstall and the files will be overwritten. I never recommend uninstalling/reinstalling PBP except as a last resort.